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w6 4 dc mass spect gold serum golden west diagnostics  (Golden West Biologicals)


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    Golden West Biologicals w6 4 dc mass spect gold serum golden west diagnostics
    W6 4 Dc Mass Spect Gold Serum Golden West Diagnostics, supplied by Golden West Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/w6+4+dc+mass+spect+gold+serum+golden+west+diagnostics/Mass+Spect+Gold+Human+Serum%2C+Ultra-Low+Hormones+%26+Steroids/pm30566883-207-240-246
    Average 93 stars, based on 15 article reviews
    w6 4 dc mass spect gold serum golden west diagnostics - by Bioz Stars, 2026-09
    93/100 stars

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    Lysis:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Reverse Transcription:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Electron Microscopy:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Recombinant:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Staining:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Isolation:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Sequencing:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2

    Single Photon Emission Computed Tomography:

    Article Title: Commensals Suppress Intestinal Epithelial Cell Retinoic Acid Synthesis to Regulate Interleukin-22 Activity and Prevent Microbial Dysbiosis.
    Article Snippet: Reagents or Resource Source Identifier Collagenase VIII Sigma-Aldrich Cat#: C2139-100MG DNase I Sigma-Aldrich Cat#: 4536282001 Dispase Sigma- Aldrich Cat#: D4848-2MG Isoflurane Piramal Healthcare NDC 66794-013-25 Fetal Bovine Serum (FBS) Genesee Scientific Cat#: 25-514H Trans- Retinoic Acid Sigma-Aldrich Cat#: 302-79-4 Percoll Sigma-Aldrich Cat#: GE17-0891-01 Eosin-Y Fisher HealthCare Cat#: 22220104 Hematoxylin Fisher Cat#: 22220102 Methyl Green Fisher Scientific Cat#: 7114-03-6 Dimethyl Sulfoxide Fisher Scientific Cat#: 67-68-5 Methacarn Fisher Scientific Cat#: NC0547175 Bovine Serum Albumin (BSA) Fisher Scientific Cat#: BP9706100 RIPA Lysis Buffer Millipore Sigma Cat#: 20-188 Tween-20 Fisher Scientific Cat#: 900-64-5 M-MLV Reverse Transcriptase ThermoFisher Scientific Cat#: 28025013 Fluoro Gel (With Tris Buffer) Electron Microscopy Sciences Cat#: 17985-10 Trans- Retinoic Acid Sigma Aldrich Cat#: 554720 IL-22 recombinant Protein Genentech IL22-Fc, Genentech PRO312045 Critical Commercial Assay Foxp3 Staining Buffer Set Kit eBioscience Cat#: 00-5523-00 RNeasy Mini Kit QIagen Cat#: 74106 RNAqueous TM- Micro Total RNA Isolation Kit Thermo Fisher Scientific Cat#: AM1931 iScript RT Mix Bio-Rad Cat#: 1708841 Deposited Data Sequencing data NCBI Sequence Read Archive (SRA) BioProject: PRJNA400781 (https://www.ncbi. nlm.nih.gov/bioproject/?term=PRJNA400781) Retinoid Quantification Reagents All-trans-retinoic acid Sigma-Aldrich Cat#: R2625 13-cis-retinoic acid Sigma-Aldrich Cat#: R3255 All-trans-retinyl palmitate Toronto Research Chemicals Cat#: R275450 All-trans-retinol Toronto Research Chemicals Cat#: R252000 All-trans-retinoic acid-d5 Toronto Research Chemicals Cat#: R250202 13-cis-retinoic acid-d5 Toronto Research Chemicals Cat#: R250004 All-trans-retinyl palmitate-d4 Cambridge Isotope Laboratory Cat#: DLM-4902-PK All-trans-retinol-d8 Cambridge Isotope Laboratory Cat#: DLM-9306-PK Formic Acid Fisher Scientific Cat#: A117-50 Acetonitrile (LC-MS grade) Fisher Scientific Cat#: A996-4 Water (LC-MS grade) Fisher Scientific Cat#: W6-4 DC MASS SPECT GOLD serum Golden West Diagnostics Cat#: MSG4000 Hexanes Fisher Scientific Cat#: H292-4 Hydrochloric acid Fisher Scientific Cat#: A144SI-212 Potassium hydroxide Fisher Scientific Cat#: P250-500 Sodium chloride Fisher Scientific Cat#: S271-1 Ethanol 200 proof Decon Labs Cat#: 2701 Ascentis RP-amide column (2.7mm, 15cm x 2.1 mm) Sigma-Aldrich Cat#: 53914-U Ascentis RP-amide Guard Cartridge (2.7 mm, 5 mm x 2.1 mm) Sigma-Aldrich Cat#: 53514-U (Continued on next page) Immunity 49, 1103–1115.e1–e6, December 18, 2018 e2



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